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Carotenoids, retinoids modulate ovarian development in Litopenaeus vannamei

J. Paniagua-Michel, Ph.D. M. Liñan-Cabello, Ph.D.

Can protect reproductive tissue from toxic levels of free oxygen radicals

Carotenoids
In this study, different concentrations of carotenoids and retinoids were administered by injection to female L. vannamei.

Functional metabolites like caro-tenoids are bioactive ingredients with physiological or health benefits beyond basic nutritional functions. The levels of carotenoids in commercial shrimp feeds are generally insufficient under intensive culture conditions to reach the physiological levels needed for critical bioactive functions in shrimp. As a result, the animals may lose their natural capacity for metabolic bioconversion to more bioactive and functional forms, such as retinoids.

The consequences include a decrease in their immunoresistance, development of diseases, and interruption of critical processes like maturation and reproduction. A study evaluated the bioactive nonpigmenting functions of carotenoids and retinoids in reproductive organs during maturation of the white shrimp (Litopenaeus vannamei).

Carotenoids and retinoids 

Carotenoids are nonproduced de novo metabolites and natural components in the diet of shrimp and many other marine organisms. Once ingested by shrimp, carotenoid molecules are unstable and chemically reversible.

Retinoids are a class of molecules derived from vitamin A considered crucial for many growth, development, and homeostatic processes in animals. They are considered to be hormone-like compounds. However, the functions of retinoids in penaeid shrimp have not been sufficiently studied.

According to the metabolic pathway of crustaceans, carotenoids can derive into other metabolites such as retinoids. The study determined that carotenoids are natural precursors of retinoids in L. vannamei.

Study setup

In this study, different concentrations of beta-carotene (18.6 μg per gram), astaxanthin (18.6 μg per gram), retinal palmitate (5.3, 10.6, 16.0 μg per gram) and all-trans retinoic acid (133, 266, 400 μg per gram) were administered by injection to farmed, reproductively exhausted female L. vannamei females in gonadic conditions II and IV, respectively. The shrimp had been formerly eyestalk ablated.

Results

After the experimental period, carotenoids, retinoids, and their respective derived metabolites were analyzed. Immunohistochemistry was also performed to detect receptors of retinoids. Table 1 shows the effects of the carotenoids and retinoids administered.

Paniagua-Michel, Survival and average value of gonodosomatic (GSI) and hepatosomatic (HSI) indexes, Table 1

TreatmentSurvival (%)GSIHSI
Beta-Carotene1002.51 ± 0.192.16 ± 0.16
Astaxanthin1003.05 ± 0.342.23 ± 0.22
Retinoic acid 1890.98 ± 0.342.13 ± 0.06
Retinoic acid 2641.40 ± 0.512.27 ± 0.40
Retinoic acid 3640.99 ± 0.232.12 ± 0.34
Retinol palmitate 11003.24 ± 0.422.24 ± 0.57
Retinol palmitate 21003.69 ± 0.492.42 ± 0.45
Retinol palmitate 31004.70 ± 0.462.24 ± 0.43
Control1001.70 ± 0.332.40 ± 0.38
Table 1. Survival and average value of gonodosomatic (GSI) and hepatosomatic (HSI) indexes in L. vannamei broodstock under experimental conditions (n = 13).

 

It was determined that retinoic acid had a teratogenic effect in the shrimp. Results showed the treatment based on retinol palmitate significantly enhanced (p < 0.05) gonadic maturation and survival in first term, with 40 percent of the population reaching maturation by day 5 of treatment. The study also showed that astaxanthin and beta-carotene play an important role not only in modulating maturation, but also as precursors of retinoids at different levels of bioactivity.

New metabolites

The first registered presence in L. vannamei of isomers 13-cis retinol, 9-cis retinol, all-trans retinal, and all-trans retinol indicated functional roles as effectors of maturation and reproduction. Results indicated the bioconversion of carotenoids into retinoids occurs in L. vannamei, and that both oxygenic carotenoids (mainly astaxanthin and others involved in shrimp metabolic pathways) and anoxygenic carotenoids (mainly beta-carotene) must be supplied at physiological levels in the diet to achieve pigmentation and bioactive functions such as full conversion to retinoids.

Proposed model

Carotenoids
Fig. 1: Bioactive roles of dietary carotenoid and retinoids in a comparment model (main organs) for ovarian maturation and reproduction of Litopenaeus vannamei. XO-SG = X organ-sinus gland complex.

Fig. 1 shows the proposed model of bioconversion of dietary carotenoids into retinoids in the organs involved in the maturation of penaeid shrimp. Different carotenoids show different degrees of bioconversion to retinoids in shrimp. Oxygenic carotenoids exhibit less bioconversion properties, while anoxygenic carotenoids are readily bioconverted to two molecules of retinoids. The uptake of dietary carotenoids is bioconverted to retinoids in the digestive gland and carried in carotenolipoproteins in the haemolymph to the gonads, where more specialized retinoids are produced.

In some crustaceans and other arthropods, isomers of retinoids bind to hormonal nuclear receptors. These receptors are known to play important functions as signaling factors for protein and cellular differentiation.

Conclusion

Results of this study reinforced the need for inclusion of carotenoids in commercial shrimp diets and showed that in L. vannamei, retinoid metabolites induce growth and development by controlling the production of local morphogenic signals by a nuclear receptor signaling pathway, which enhances maturation and associated processes. At this level, carotenoids play a role in protecting reproductive tissue from toxic levels of free oxygen radicals, and as a reserve during embryogenesis and development.

(Editor’s Note: This article was originally published in the October 2002 print edition of the Global Aquaculture Advocate.)


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