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Dietary threonine factor in tilapia fillet yield

Dr. Wilson Massamitu Furuya Mariana Michelato Dr. Valéria R. Barriviera Furuya

Can be used to maximize fish growth, feed efficiency, meat production

threonine
Involved in protein synthesis, dietary threonine has a high influence on fish growth and fillet yield.

Tilapia culture has played an important role in the economy of Brazil, changing from traditional culture in earthen ponds to more intensive culture in cages. It is estimated that approximately 150,000 metric tons per year of this species are cultured in Brazil, making the country the most important producer in South America.

When fish are reared at high densities, nutritionally complete diets are necessary to support growth performance and fish health. Recently, tilapia nutrition and feeding – particularly the importance of amino acid nutrition for tilapia under intensive culture – have received special attention by many research groups in Brazil.

Fishmeal production has remained stable, and alternative ingredients such as feather meal, blood meal, and meat and bone meal protein have been used as substitutes for fishmeal in commercial diets for Nile tilapia in Brazil. However, the apparent digestibility coefficients for protein and amino acids in these ingredients are lower when compared to values for soybean meal.

Threonine

Threonine is the major component of mucin in the small intestines and skin of tilapia, and its supplementation is important for maintenance, growth and fish health. The threonine requirement for maintenance is high compared to other amino acids due to its high concentrations in intestinal and skin secretions. For this reason, threonine is very important for large fish, because the requirement for maintenance is much higher.

Threonine has an important influence on skeletal muscle, where it can change myoblast proliferation and differentiation, the composition of cytoplasmic organelles, the number and diameter of white and red muscle fibers, and patterns of gene expression related to muscle development. Involved in protein synthesis, dietary threonine has a high influence on fish growth and fillet yield.

Growth trial

To evaluate the effects of dietary threonine under commercial conditions, a trial was set up in the Paranapanema River in Brazil. Nile tilapia of 563.3 ± 15.9-g initial weight were randomly stocked into 20, 1,000-L net cages at 30 fish/cage and fed extruded isocaloric (3,043 kcal digestible energy/kg) and isoproteic (260 g/kg of digestible protein) diets containing digestible threonine values of 8.96, 10.58, 12.20, 13.83 and 15.44 g/kg. Fish were hand fed three times daily to apparent satiety for 30 days.

Results

Survival at the end of the experiment was about 98 percent, and no difference was observed among treatments. No statistically significant differences in daily weight gain and feed conversion were observed.

The dietary threonine:lysine ratios affected net protein utilization, with the best value at 11.54 g/kg of threonine (Table 1). For fillet weight and fillet yield, the maximum values were estimated at 11.43 and 11.45 g/kg of threonine, respectively (Figures 1 and 2). Diets containing 8.96-15.44 g/kg of threonine did not affect fish growth, feed conversion and survival. However, for maximal meat production of Nile tilapia from 550 to 800 g, the dietary threonine requirement is 11.40 g/kg.

Furuya, Mean values of Nile tilapia, Table 1

ParameterDietary Threonine (g/kg)
8.96
Dietary Threonine (g/kg)
10.58
Dietary Threonine (g/kg)
12.20
Dietary Threonine (g/kg)
13.83
Dietary Threonine (g/kg)
15.44
Standard ErrorP Value
Initial weight (g)558.75565.5566.5572.25553.503.26≥ 0.05
Final weight (g)783.74829.31786.79769.96761.293.34≥ 0.05
Daily weight gain (g)7.498.797.346.596.930.03≥ 0.05
Feed-conversion ratio1.471.261.511.681.600.07≥ 0.05
Net protein utilization (%)41.9450.8446.5739.5842.072.010.036
Table 1. Mean values of Nile tilapia performance fed graded levels of digestible threonine.
threonine
Fig. 1: Fillet weight of Nile tilapia fed graded levels of dietary threonine.
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Fig. 2: Fillet yield of Nile tilapia fed graded levels of dietary threonine.

Perspectives

The use of crystalline amino acids in diets for tilapia must be based on the global benefits on fish growth, meat production and economic viability. For fillets, the most important product from tilapia aquaculture, L-threonine should be used to maximize fish growth, feed efficiency and meat production. Individual amino acids digestibility values should be determined for each ingredient to establish properly balanced diets for the sustainable and competitive growth of world tilapia production.

(Editor’s Note: This article was originally published in the July/October 2013 print edition of the Global Aquaculture Advocate.)