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Finishing feeds tailor fatty acid composition in tilapia fillets

Jesse T. Trushenski, Ph.D. John Boesenberg Christopher C. Kohler, Ph.D.

Wholesale conversion to alternative formulations compromises nutritional value

Finishing feeds
Feeding tilapia with diets containing alternative oils resulted in the accumulation of the oils’ constitutive fatty acids within the fillets.

Historically, aquafeeds were comprised largely of marine-derived ingredients such as fishmeal and fish oil. The rising cost of these products and public pressure from environmental and human food safety groups have created incentives to reformulate aquafeeds with greater levels of alternative proteins, fats and oils.

In a recent State of World Fisheries and Aquaculture report, the Food and Agriculture Organization concluded: “Given the difficulty in replacing fish oils … it is clear that competition for fish oil is likely to be a more serious obstacle for some sections of the aquaculture industry.” While direct fishmeal replacement has also proven difficult, fish oil replacement is considered more difficult, in part because alternative sources of the nutrients found in fish oil are lacking. The essential amino acids in fishmeal are ubiquitous, albeit not always in the desired proportions. The essential fatty acids found in fish oil are not so abundant.

Fish oil in aquaculture

Fish oil contains high levels of long-chain polyunsaturated fatty acids (LC-PUFAs), such as eicosapentaenoic acid and docosahexaenoic acid, which are known to have positive impacts on a variety of human health disorders and conditions. Excluding specialty oils derived from algal or fungal cultures, fish oil alternatives contain, at most, trace levels of these nutrients.

Because the fatty acid composition of fish tissue is influenced by dietary fatty acid profile, fillets of fish raised on feeds with reduced or no fish oil contain substantially lower levels of beneficial LC-PUFAs. Herein lies the conflict: The need to reduce reliance on fish oil is clear, but none of the commercially viable alternatives provide equivalent levels of LC-PUFAs, and wholesale conversion to alternative formulations compromises the nutritional value of farm-raised seafood.

Finishing strategies

To reduce fish oil use while maintaining product quality, producers can use “finishing” strategies to restore fillet LC-PUFA content in fish raised on alternative feeds. Finishing strategies divide the production cycle into growout and finishing periods, which utilize different feeds.

During grow-out, fish are fed alternative fat- or oil-based feeds. At a predetermined time before harvest, they are switched to a fish oil-based feed for finishing and restoration of fillet fatty acid profile.

Finishing strategies have been used to improve fillet quality in a number of fish. However, the extent of LC-PUFA enrichment achieved varies. The authors have noted greater finishing success in hybrid striped bass when the growout feed contained higher levels of saturated and monounsaturated fatty acids (SFAs and MUFAs), typically associated with animal fats and certain plant oils, as opposed to the medium-chain polyunsaturated fatty acids (MC-PUFAs) typically associated with grains and oilseeds.

Grow-out trial

In order to determine whether this was also the case for Nile tilapia, the authors assessed finishing success among Oreochromis niloticus raised on grow-out feeds containing a 50/50 blend of fish oil and poultry (MUFA-rich), linseed (n-3 MC-PUFA-rich), grapeseed (n-6 MC-PUFA-rich) or coconut (SFA-rich) oils.

Replicate tanks of tilapia were raised in a recirculation system for 11 weeks, after which half of the tanks within each dietary group were switched to a 100 percent fish oil based feed for four weeks of finishing. The remaining tanks were kept on their assigned, reduced fish oil-feeds for the rest of the trial. A control group of fish was raised exclusively on the 100 percent fish oil feed.

Finishing success

After 15 weeks of culture, the mean survival (95 ± 3 percent), weight gain (205 grams ± 8 percent), feed-conversion ratio (1.9 ± 0.1) and dress-out (87.6 ± 0.3 percent, eviscerated carcass) of the tilapia were unaffected by the various feeding strategies, indicating this species could effectively utilize coconut, linseed, grapeseed, poultry and fish oils. Feeding the alternative oils resulted in the accumulation of their constitutive fatty acids within the fillets, particularly MUFAs and MC-PUFAs (Fig. 1).

tilapia
Fig. 1: Lipid composition of tilapia fillets with respect to dietary lipid sources. Solid bars represent unfinished treatments. Hatched bars represent groups that received a four-week finishing prior to harvest. Bars with common superscripts are not significantly (P < 0.05) different.

These departures from a fish oil-associated profile were largely corrected by finishing, but profile restoration was most complete among fish fed the coconut and poultry oil-based feeds during grow-out. Although differences were not significant in all cases, levels of fish oil-associated fatty acids were elevated among finished groups relative to their unfinished counterparts.

Perspectives

A number of issues may have contributed to the authors’ inability to resolve statistical significance for all the fatty acids and fatty acid groupings. First, they observed a high level of individual-to-individual variation in this study, likely because of the errant reproduction that occurred during the feeding trial. Additional lipid nutrition studies using all-male populations of Nile tilapia are currently under way to address this issue.

Second, fillet levels of LC-PUFAs were relatively well maintained regardless of feeding strategy, which may have weakened the influence of finishing on LC-PUFA augmentation. This suggested Nile tilapia have considerable ability to synthesize LC-PUFAs from shorter-chain fatty acid precursors and/or conserve LC-PUFAs within their tissues when dietary levels are reduced. Further research is needed to address these issues.

Editor’s Note: This article was based on a paper by the authors recently published in the North American Journal of Aquaculture: “Influence of grow-out feed fatty acid composition on finishing success in Nile tilapia.”

(Editor’s Note: This article was originally published in the September/October 2009 print edition of the Global Aquaculture Advocate.)


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