A new direction in our understanding of the cause of deformities
Dietary phosphorus is essential for growth and healthy skeletal development of farmed fish. Historically, fishmeal has been the key source of phosphorous in fish feeds; however, sustainable aquaculture increasingly demands diets that contain little or no fishmeal. In modern fish diets, inorganic phosphorus is an efficient and reliable replacement for fishmeal. Inorganic phosphorous is, however, a limited resource on the planet and the growing aquaculture industry will have to compete with agriculture for this important mineral. Fish health, limited availability and environmental concerns all urge modern aquaculture to handle phosphorous resources wisely and sustainably.
The experimental approach
An important component of sustainable phosphorous management is to understand the role of phosphorous for healthy skeletal development. Several types of skeletal malformations are believed to be caused by an insufficient dietary phosphorous supply. However, previously it has not been possible to quantify the risks and to establish if malformations are a direct or an indirect result of phosphorus deficiency. In our experiments described in the full report (see below) and led by Dr. Eckhard Witten from Ghent University together with Dr. Matthew Owen from the Skretting Aquaculture Research Centre, the effects of feeding Atlantic salmon with a diet that was severely deficient in phosphorus were examined. The experimental diet contained only half of the amount of phosphorus thought to be required for healthy development.
A surprising result
At the conclusion of the experimental period, it was expected that there would be several spinal malformations from a list of pathologies previously published (Aquaculture 2009, 295:6-14). Instead, all animals in the experimental group and in the control group had perfectly straight spines. However, the comparison with x-rays of spines from control animals showed smaller and more widely spaced vertebral bodies in the fish fed the low phosphorus diet (Figure 1).
Following this finding, mineralized (non-decalcified) bone samples were subjected to microscopic analysis. Surprisingly, vertebral bodies of Atlantic salmon that received the phosphorous deficient diet were not as small as suggested by the x-rays. Bone and also scale growth continued in a regular fashion. It became apparent that mineralization of the bone stops soon after the animals were put onto a phosphorous-deficient diet. Still, bone growth continued and there were no signs of resorption or malformation of the non-mineralized bone (Figure 2).
Future fish diets and phosphorus management
Our research shows for the first time how severe primary phosphorus deficiency affects the salmon skeleton at the cellular level. The 10-week period of the experiment generated a homogeneous phosphorus-deficient skeleton but no apparent malformations. It can be surmised that bone formation and bone mineralization are, to a large degree, independent. Phosphorus deficiency does not immediately cause malformations. Moreover, phosphorus deficiency alone and without additional adverse factors is perhaps not the cause of malformations. This research continues and experiments have been launched that will answer the open questions about the types of deformities that may eventually arise.
A refined understanding of the role of phosphorus for healthy skeletal development will help the growing aquaculture industry to manage the important and valuable feed ingredient phosphorus and to use it in a sustainable manner.
Authors’ note: A full report is available as an “open access” publication: Witten PE, Owen MAG, Fontanillas R, Soenens M, McGurk C, Obach A (2016). Primary phosphorous-deficiency in juvenile Atlantic salmon: the uncoupling of bone formation and mineralization. Journal of Fish Biology, in press.