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Saturated fatty acid-rich soybean oil spares fish oil in sea bass feed

Jesse Trushenski, Ph.D. Bonnie Mulligan David Jirsa Mark Drawbridge

Omega-3 requirements could be effectively reduced via SFA-rich alternative lipids

sea bass
Test data suggested that the LC-PUFA requirements of white sea bass feed could be effectively reduced by feeding SFA-rich alternative lipids like hydrogenated soybean oil.

Fish oil sparing has proven particularly difficult for fish species that require one or more of the long-chain polyunsaturated fatty acids (LC-PUFAs), which are abundant in fish oil, but lacking in most of the alternative fats and oils commonly used in aquafeeds. Carnivorous fish, especially marine carnivores like the white sea bass (Atractoscion nobilis) are particularly demanding in this context. For these species, complete or near-complete fish oil replacement generally impairs growth performance and dramatically alters the fatty acid composition of the tissues.

Recent studies have suggested that the use of lipids rich in saturated fatty acids (SFAs) instead of those rich in C18 polyunsaturated fatty acids (C18 PUFAs) may be advantageous in maintaining tissue levels of LC-PUFAs. SFA-rich lipids may also offer a strategic advantage in terms of meeting the LC-PUFA requirements of marine carnivores while minimizing dietary fish oil inclusion.

Initial feeding trial

Accordingly, the authors assessed the performance and tissue fatty acid composition of juvenile white sea bass initially weighing 3.8 + 0.2 g fed diets containing fish oil, graded levels of C18 PUFA-rich standard soy oil or SFA-rich hydrogenated soy oil (replacing 25, 50, 75, or 100 percent of dietary fish oil) for eight weeks.

Feed conversion, weight gain and specific growth rates were not impaired by partial or complete replacement of dietary fish oil with hydrogenated soy oil. However, fish oil sparing with standard soy oil was associated with declining performance. Tissue fatty acid profiles of fish fed the SFA-rich, hydrogenated soy oil-based diets were very similar to those fed the fish oil-based feed, but the C18 PUFA-rich standard soy oil-based feeds resulted in concomitant loss of n-3 and LC-PUFAs (Fig. 1).

seabass
Fig. 1: Fatty acid composition of fillet total lipid from white seabass fed diets containing graded levels of C18 PUFA-rich, standard soybean oil (STD SOY) or SFA-rich, hydrogenated soybean oil (SFA SOY) expressed as a fraction of fatty acid composition observed in fish fed the LC-PUFA-rich, menhaden fish oil-based control diet. A value of 1 represents equality between tissue profiles. Only fatty acids or fatty acid groupings representing over 2 percent of total fatty acid methyl esters quantified are shown.

These data suggested a limitation, potentially related to LC-PUFA deficiency, associated with replacing fish oil with standard soybean oil but not with hydrogenated soybean oil.

Follow-up trial

In a subsequent feeding trial, the authors tested whether the limitations associated with feeding standard soybean oil to white sea bass could be attenuated by the addition of an algal source of docosahexaenoic acid (22:6 n-3, DHA). This experiment assessed the performance and tissue fatty acid compositions of juvenile white sea bass weighing 4.1 g fed diets containing fish oil, C18 PUFA-rich standard soy oil or SFA-rich hydrogenated soy oil as a complete fish oil replacement, with or without DHA supplementation for eight weeks. Once again, the use of C18 PUFA-rich standard soybean oil-based feed resulted in impaired growth fish and feed-conversion ratios. Although amending the diet with DHA did improve the performance of fish fed standard soybean oil-based feed, growth was still superior among the fish fed hydrogenated soybean oil-based feeds, regardless of DHA supplementation.

Perspectives

Collectively, the data suggested that the LC-PUFA requirements of white sea bass could be effectively reduced by feeding SFA-rich alternative lipids like hydrogenated soybean oil, allowing for a greater level of fish oil sparing without growth impairment or tissue profile modification than is possible with C18 PUFA-rich lipids.

To fully validate these results and the value of hydrogenated soybean oil-based white seabass feed, a commercial-scale grow-out trial is currently being conducted by Hubbs-SeaWorld Research Institute in cooperation with researchers at Southern Illinois University and Iowa State University.

Editor’s Note: This article was based largely on research by the authors reported in “Sparing Fish Oil With Soybean Oil in Feeds for White Seabass: Effects of Inclusion Rate and Soybean Oil Composition,” an article published in the April 2013 North American Journal of Aquaculture.

(Editor’s Note: This article was originally published in the November/December 2014 print edition of the Global Aquaculture Advocate.)