News features and technical articles about the evolution of aquaculture, one of the world’s fastest growing industries.

Spirulina meal, feeding attractant spare fishmeal in white shrimp diets

Marcelo V.C. Sá, Ph.D. Alberto J.P. Nunes, Ph.D. José Fernandes Silva-Neto, M.S. Hassan Sabry-Neto, M.S.

With effective feeding attractant, possible to reduce fishmeal content as much as 25 percent

Spirulina
A trial at LABOMAR compared the effects of spirulina meal (left) and a commercial feeding attractant in sparing fishmeal in diets for white shrimp.

Most feeding effectors described for penaeid shrimp are of animal marine origin, although some work has indicated that spirulina, a genus of filamentous microalgae, has high attractiveness for Southern white shrimp (Litopenaeus schmitti). Spirulina are frequently found in tropical and salty waters with alkaline pH.

Spirulina can double biomass within three to five days, with a growth rate comparable only to yeast and bacteria. Under culture conditions, annual yields can reach 25 metric tons (MT) or 15 MT of crude protein per hectare. Currently, 22 companies around the world produce spirulina biomass, which is marketed primarily as supplements to improve human health.

The authors recently investigated whether low inclusion levels of a commercial feeding attractant (bivalve mollusk biomass supplemented with synthetic amino acids) and spirulina meal can spare fishmeal in complete diets for Pacific white shrimp (Litopenaeus vannamei).

LABOMAR trial

Twelve-day old L. vannamei postlarvae were obtained from a commercial shrimp hatchery and transported by road to aquaculture facilities at the Instituto de Ciências do Mar (LABOMAR) in northeastern Brazil. The shrimp were reared for 45 days in 3,000-L nursery tanks to 1.5 g body weight. After two additional weeks in 1,000-L outdoor tanks, juveniles of 3.89 ± 0.25 grams were stocked in 500-L tanks at 44 shrimp/tank or 77 juveniles per square meter and reared for 10 weeks.

Seven isonitrogenous and isoenergetic diets were formulated. There were three control diets (STD, N25 and N50) and four experimental diets (C25, S25, C50 and S50). STD was the positive control in which regular levels of fishmeal and soybean meal were used (Table 1). STD was also supplemented with a reliable feeding attractant for shrimp at 0.5 percent. This was a commercial product containing the amino acids alanine, valine, glycine, proline, serine, histidine, glutamic acid, tyrosine and betaine with enzymatically digested bivalve mollusk.

The negative-control diets N25 and N50 had 25 and 50 percent lower fishmeal inclusion than in STD. No feeding attractant was added to the diets. Experimental diets C25 and C50 were supplemented with 0.5 percent of the feeding attractant, while S25 and S50 had 0.5 percent Spirulina meal. In diets C25 and S25, fishmeal was reduced 25 percent. C50 and S50 had 50 percent of the STD diet’s fishmeal content (Table 1).

Sa, Ingredient and nutritional composition, Table 1

IngredientComposition (%, as fed)
STD
Composition (%, as fed)
N25
Composition (%, as fed)
N50
Composition (%, as fed)
C25
Composition (%, as fed)
S25
Composition (%, as fed)
C50
Composition (%, as fed)
S50
Peruvian fishmeal18.5013.879.2413.8713.879.249.24
Soybean meal25.0027.4735.4427.8627.0835.4435.02
Poultry by-product meal8.0010.0010.0010.0010.0010.0010.00
Corn gluten meal4.004.913.003.784.843.003.00
Wheat flour13.2617.4515.2217.6517.4115.2215.17
Broken rice15.2710.0010.0010.0010.0010.0010.00
Fish oil4.184.144.414.184.154.414.41
Others*4.154.154.154.154.154.154.15
Bicalcium phosphate2.713.013.553.013.003.553.54
Spirulina meal00000.5000.50
Commercial attractant**0.50000.5000.500
Bentonite4.435.004.995.005.004.494.97
Nutritional Composition (%, dry- matter basis)
Crude protein36.5636.3435.3435.8636.1336.1035.93
Ether extract9.809.879.569.569.579.669.89
Ash14.0814.5714.6414.3414.2714.1114.47
Lysine2.051.991.991.981.981.991.99
Methionine0.760.720.660.710.730.660.66
Gross energy (kJ/g)17.4317.2517.9718.0417.8617.80
* 2.00% soybean lecithin, 0.15% cholesterol, 0.50% common salt, 1.00% mineral-vitamin premix,
0.50% synthetic binder.
** Complex of amino acids with enzymatically digested bivalve mollusk.


Table 1. Ingredient and nutritional composition of the control and experimental diets.

An entirely randomized design was adopted in this study with three controls and four treatments, each with four tanks. Shrimp were fed on demand twice a day over the complete rearing period.

For the feed preference trial, shrimp of 12.00 ± 0.65 grams were stocked in 24, 500-L tanks at 20 animals/tank or 35 shrimp per square meter. Shrimp were fed once daily to excess with two selected diets delivered separately in equal amounts from feeding trays located on opposite sides of each tank bottom. Diets were delivered at 7:30 a.m. and remains collected at 3:30 p.m. Feed preference was measured over one week by determining the amount of dried feed remains in each tray.

The following diets were compared against each other: C25 and S25, C50 and S50, STD and S25, STD and S50, N25 and S25, and N50 and S50.

Results

At harvest, final shrimp survival was above 89 percent for all diets. Significant (P < 0.05) differences in survival were observed between shrimp fed diets C25 (95.5 percent), N50 (89.8 percent) and C50 (87.5 percent). Likewise, feed-conversion ratios were significantly higher for shrimp fed N50 (3.07) than those in all other treatments.

There was a clear detrimental effect on the growth performance of L. vannamei when fishmeal was withdrawn from the diets without supplementation of the commercial attractant or spirulina meal. Shrimp fed the negative control diets with reduced fishmeal achieved significantly (P < 0.05) lower body weights and slower growth than animals fed the STD diet (Table 2).

Sa, Performance of juvenile L. vannamei , Table 2

DietFinal Body Weight (g)Weekly Growth (g)Survival (%)Feed-Conversion RatioFeed Intake (g/tank)
STD13.22 ± 0.90a0.95 ± 0.08a89.0 ± 0ab2.61 ± 0.29a903.0 ± 14.1
N2512.25 ± 0.45b0.82 ± 0.06b93.8 ± 1.1ab2.83 ± 0.16a926.0 ± 14.7
N5012.29 ± 0.32b0.83 ± 0.02b89.8 ± 2.3b3.07 ± 0.21b948.0 ± 10.8
C2512.83 ± 0.27ab0.89 ± 0.01ab95.5 ± 4.2a2.50 ± 0.10a911.0 ± 29.8
S2512.80 ± 0.46ab0.89 ± 0.03ab94.9 ± 2.9ab2.55 ± 0.08a928.0 ± 14.0
C5013.05 ± 0.54ab0.92 ± 0.06ab87.5 ± 7.1b2.75 ± 0.28a912.0 ± 29.4
S5012.30 ± 0.50b0.85 ± 0.06b90.9 ± 4.2ab2.83 ± 0.19a918.0 ± 22.3
P0.007< 0.0010.0370.008> 0.05
Means with different letters in the same column are statistically different.


Table 2. Performance of juvenile L. vannamei fed diets with reduced levels of fishmeal, supplemented with Spirulina meal or a commercial feeding attractant.

On the other hand, inclusion of the feeding attractant counteracted the reduced levels of fishmeal in diets C25 and C50. Shrimp final body weight and weekly growth for these diets varied 12.8 to 13.1 g and 0.89 to 0.92 grams, compared with 13.2 and 0.95 grams achieved with the control diet STD.

Similarly, the inclusion of spirulina meal allowed reduction of fishmeal up to 25 percent with no detrimental effect on shrimp growth performance. Shrimp fed the S50 diet containing 0.5 percent spirulina meal and 9.2 percent fishmeal achieved a similar final weight and weekly growth comparable to those for shrimp fed the negative control diet with 13.9 percent fishmeal.

The feed preference trial indicated that the diet S25 with a 25 percent reduction in fishmeal and 0.5 percent spirulina meal was preferred over the C25 diet with 0.5 percent attractant (Fig. 1). At a 50 percent reduction in fishmeal, no difference was observed in the amount of leftover feed between diets C50 and S50.

feed
Fig. 1: Mean amounts of daily feed remains collected from feeding trays after eight hours in water. Bars with an asterisk are significantly different (P < 0.05)

Supplementation with Spirulina meal was able to elicit a stronger feed intake in shrimp when fishmeal was reduced by 50 percent in the negative control diet N50. Also, shrimp feed intake was not significantly reduced when the STD diet was compared to the S25 diet with 25 percent less fishmeal and supplemental Spirulina meal.

Perspectives

Results from present work indicated it is possible to reduce fishmeal content as much as 25 percent in a complete diet for L. vannamei without deleterious effects on shrimp growth performance as long as an effective feeding attractant is used in the diet.

The authors’ work also found that at moderate levels of fishmeal (13.9 percent), spirulina meal elicited shrimp feeding responses as efficiently as a commercial attractant with marine animal origins and a complex of amino acids.

The commercial feeding attractant was superior to spirulina meal in stimulating L. vannamei feed intake. Nevertheless, it is noteworthy that while the attractant used contained many different compounds, spirulina meal is formed by a single raw material with known chemical composition.

(Editor’s Note: This article was originally published in the May/June 2011 print edition of the Global Aquaculture Advocate.)