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Microbound diet proves successful as replacement for artemia nauplii

Louis R. D’Abramo, Ph.D.

Successful diet developed for freshwater prawns

microbound diet
A stage 6 larva of Macrobrachium rosenbergii grasps the microbound diet while eating it. Note the stomach (below eyes) is completely full of the orange-colored diet, which contains carotenoids. Photo by Ekaterina Kovalenko.

During the past five years, the Mississippi Alabama Sea Grant Consortium funded a research project by the author to develop a larval diet that would replace artemia as food for larval fish and crustaceans. After numerous modifications during experiments with the Malaysian freshwater prawn (Macrobrachium rosenbergii), a successful diet has been developed.

Artemia replacement

The ultimate goal of the project was to produce a formulated diet that would serve as a complete replacement for live food by achieving similar production characteristics at lower cost.

Cyst availability

The culture of larvae of many species of fish and crustaceans is precariously dependent upon the availability of artemia cysts from which the food, artemia nauplii, is obtained through hatching. The availability of these cysts from the natural environment is subject to periodic, unpredictable shortages. As a result, demand cannot always be supplied, and prices increase. The hatching of cysts is labor-intensive and also adds operational costs.

Nutritional quality

In addition, variations in the nutritional quality of hatched artemia nauplii relative to season and locale of harvest have been a chronic problem. This has been partly overcome by methods to enhance the nutritional value of the artemia, particularly as it relates to the content of two highly unsaturated fatty acids, docosahexaenoic acid and eicosahexaenoic acid.

Previous diets

Past attempts to produce nutritionally complete larval diets are commonly characterized as microencapsulated, microbound, or microcoated diets. To date, they have had limited success and, in most cases, not served as complete artemia substitutes.

Most of the success has been with herbivorous forms of larvae, where the rapid transit time through their guts did not require a highly digestible diet. Lack of success with carnivorous larvae was attributed to a long gut retention time and the possible absence of sufficient digestive enzymes during early development due to the lack of a stomach.

Initial attempts in this new research met the same frustrations encountered by other investigators over the years. The prepared diets were readily consumed and the animals’ guts were filled, but growth and survival rates were far below those observed for larvae fed live artemia nauplii. These observations suggested that either or both digestion and assimilation were poor.

Enzymes 

The need for exogenous digestive enzymes in the diets was considered, but recent research strongly suggested a sufficient complement of enzymes was available during early development of fish and crustacean larvae. The inefficient digestion was probably not due to a lack of enzymes, but the quality of the enzymes relative to their capacity to effect digestion of specific protein sources.

Microbound diet

The successful microbound diet substitutes completely for live artemia nauplii during the larval metamorphosis of M. rosenbergii from stage 5 through 11 and then to postlarva. Although not experimentally documented, part of its success may be attributed to protein quality. Some protein sources have an attractive essential amino acid profile but are not efficiently digested.

Principally bound with alginate, the diet was designed to have high moisture content of about 60 percent. Presently, the high level of moisture precludes the production of particles less than 250 μ in size. However, in a dry form, the particle size can be reduced to 125 μ.

Larvae growth and survival

This is an energy-dense diet with 46 percent protein and 37 percent lipid levels. Survival of larvae fed the microbound diet exclusively for 14 days (23 days post-hatch) was 75 percent. Growth and survival of the larvae fed the microbound diet were 85 to 90 percent of that achieved with the artemia-fed larvae.

Easily modified

The most attractive characteristic of the diet is that all the ingredients are chemically defined. It does not contain any exogenous enzymes. Therefore, modification to address specific nutritional requirements of other species can be readily accomplished.

High acceptability

Another important quality of the diet is its high acceptability. In the past, experimental protocol in the testing of formulated larval diets has generally included a period of weaning from live diets. The new microbound diet does not require a weaning period. Upon first presentation in the absence of live food, this diet was immediately ingested by the M. rosenbergii larvae.

Water stability

The diet is water-stable, losing only about 5 percent of its dry weight after 1.5 hours of immersion with aeration. Most of this loss appears to be due to leaching of water-soluble nutrients. For example, 30 to 40 percent of the thiamin and riboflavin provided in a vitamin mix is lost during the first 15 minutes of immersion.

Nutrient overcompensation

microbound diet
In this dorsal view, the gastro­intestinal tract of a shrimp larva is filled with the micro­bound diet. Photo by Ekaterina Kovalenko.

To prevent the possibility of not satisfying nutritional requirements for water-soluble nutrients due to leaching, these nutrients are included in the diet at levels much higher than the reported requirements for juveniles.

This approach is particularly effective for species like Macrobrachium, which grasp particles with their feeding appendages as they ingest the diet. Some of the loss due to leaching would be reduced in the culture of carnivorous fish due to their more rapid ingestion of food as it enters the water column.

Conclusion

Although successful with M. rosenbergii, the potential of the microbound diet as an artemia substitute for other species is currently under investigation. Development and evaluation of the diet have so far been limited to small-scale conditions. The results need to be verified in commercial-size production units.

The diet should offer a final cost competitive with artemia cysts, as well as the added advantage of complete control and reproducibility of the nutrient composition of the diet through its patent-pending production process.

(Editor’s Note: This article was originally published in the August 2002 print edition of the Global Aquaculture Advocate.)


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